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TK (phospho-S13) polyclonal antibody

CAT: 0384-BS4365-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS4365-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
TK1; Thymidine Kinase; TK2
Swiss Prot
P04183
Reactivity
Human
Immunogen
Synthetic peptide derived from human TK around the phosphorylation site of S13.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TK (phospho-S13) polyclonal antibody detects endogenous levels of Thymidine Kinase protein only when phosphorylated at Ser13.
Applications Notes
Western blot (WB) analysis of p-Thymidine Kinase (S13) pAb at 1:500 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:HuT78 whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug)
Host or Source
Rabbit

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