ULK1 Antibody

• Background:

  UNC-51-like kinase 1 (ULK1) is a serine/threonine kinase broadly expressed in mammalian tissues, with a critical role in neuronal development and homeostasis. Structurally, ULK1 comprises an N-terminal kinase domain, a central proline/serine-rich region, and a highly conserved C-terminal domain. Functionally, ULK1 is a mammalian homolog of yeast Atg1 and serves as a master regulator of autophagy initiation—a process essential for cellular quality control and survival under stress. In the nervous system, ULK1 has been directly implicated in axonal growth, synaptic remodeling, and neuronal survival. Its ability to interact with multiple autophagy-related (ATG) proteins enables it to orchestrate phosphorylation-dependent signaling cascades that regulate autophagosome formation and intracellular trafficking. Dysregulation of ULK1 activity has been associated with impaired autophagic flux, a hallmark of several neurodegenerative diseases including Alzheimer’s, Parkinson’s, and Huntington’s disease. Given its dual role in autophagy and axonal dynamics, ULK1 represents a promising therapeutic target in neuroscience and neurodegeneration research. Understanding ULK1-mediated signaling pathways offers critical insights into the molecular mechanisms underlying neuronal dysfunction and opens new avenues for the development of autophagy-based interventions in neurodegenerative disorders.

• Description:

  Rabbit Anti-Human ULK1 Polyclonal

• Specifications:

  Detects ~100 kDa.

• Product Name Alternative:

  Unc-51 like kinase 1 (C. elegans), Autophagy related protein 1 homolog, Autophagy-related protein 1 homolog, FLJ38455, Serine/threonine-protein kinase ULK1, Unc51.1, ATG1A, UNC51, C. elegans, homolog of, EC:2.7.11.1, Serine/threonine protein kinase Unc51.1, FLJ46475, ATG 1, hATG1, ATG1, Unc-51-like kinase 1, Serine/threonine protein kinase ULK1, ULK1, Unc 51 like kinase 1, KIAA0722, UNC 51, ULK 1, ULK1_HUMAN, UNC51, Unc 51 (C. elegans) like kinase 1, ATG1 autophagy related 1 homolog

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  8408

• Swiss Prot:

  O75385

• Accession Number:

  NP_003556.1

• Cellular Locus:

  Cytoplasm | Cytosol | Preautophagosomal Structure

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)

• Target:

  ULK1

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Tissue Specificity:

  Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

• Type:

  Polyclonal

• Applications:

  WB | ICC/IF

• Validated Applications:

  WB, ICC/IF

• Field of Research:

  Neuroscience | Cell Signaling | Cardiovascular System | Heart | Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | Cell Signaling | Metabolism | Mitochondrial Metabolism

• Purification:

  Peptide Affinity Purified

• Detection Range:

  A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Okazaki N., et al. (2000) Brain Res Mol Brain Res. 85: 1-12. 2. Young A.R., et al. (2006) J Cell Sci. 119: 3888-900. 3. Kamada Y., et al. (2000) J Cell Biol. 150: 1507-13. 4. Lee S.B, et al. (2007) EMBO Rep. 8: 360-5. 5. Hara T., et al. (2008) J Cell Biol. 181: 497-510.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~100 kDa.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Cytosol | Preautophagosomal Structure

• Immunogen Species:

  Human