NCC Antibody

• Background:

  The Na⁺-Cl⁻ co-transporter (NCC), primarily expressed in the distal convoluted tubule of the kidney, is a thiazide-sensitive transporter critical for sodium and chloride reabsorption. While traditionally studied in renal physiology and hypertension, emerging evidence suggests that NCC may also play a role in neurodegenerative disease through its influence on systemic electrolyte balance and neuronal excitability. NCC is activated by phosphorylation and regulated by signaling pathways involving SGK1 and WNK kinases. Dysregulation of NCC activity can alter extracellular potassium and sodium levels, indirectly affecting neuronal membrane potential and synaptic transmission. Additionally, NCC expression has been detected in non-renal tissues, including the lens and possibly the brain, suggesting broader physiological roles. In neurodegenerative contexts, electrolyte imbalance and altered ion transport are known contributors to neuronal dysfunction and glial activation. NCC-mediated sodium handling may influence neuroinflammatory responses and oxidative stress, particularly in conditions such as Alzheimer’s disease and vascular dementia. Furthermore, thiazide diuretics targeting NCC have been associated with cognitive effects, underscoring the transporter’s potential relevance in brain health. Understanding NCC’s extrarenal functions may reveal novel mechanisms linking ion transport to neurodegeneration and offer new therapeutic avenues for modulating neuronal homeostasis.

• Description:

  Rabbit Anti-Rat NCC Polyclonal

• Specifications:

  Detects ~160kDa.

• Product Name Alternative:

  NCC, SLC12A3, SCYL1, CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, New CC chemokine 1, Small inducible cytokine A13, Small inducible cytokine subfamily A (Cys-Cys) member 13, Chemokine (C-C)

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  54300, 6559

• Swiss Prot:

  P55018, P55017

• Accession Number:

  NP_062218, NP_000330

• Cellular Locus:

  Membrane

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

• Target:

  NCC

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | IHC | ICC/IF | IEM

• Validated Applications:

  WB, IHC, ICC/IF, IEM

• Field of Research:

  Cancer | Neuroscience | Pumps/Transporters | Ion Pumps/Transporters

• Purification:

  Affinity Purified

• Detection Range:

  1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:200) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297 (3) : F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30 (6) : 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~160kDa.

• Species:

  Human | Mouse | Rat | Dog

• CAS Number:

  9007-83-4

• Location:

  Membrane

• Immunogen Species:

  Rat