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TLR4 Antibody

CAT: 0400-SPC-200SSize: 12 µgDry Ice: NoHazardous: No
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CAT#:0400-SPC-200SSize:12 µg
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Background
Toll-like receptor 4 (TLR4) is a transmembrane pattern recognition receptor and a central component of the innate immune system. Structurally, TLR4 is defined by extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain, which mediates downstream signaling through adaptor proteins such as MyD88 and TRIF. Among the ten known human TLRs (TLR1–TLR10), TLR4 is one of the most extensively studied, particularly for its role in recognizing lipopolysaccharide (LPS) from Gram-negative bacteria. In the central nervous system, TLR4 is expressed on microglia, astrocytes, and neurons, where it serves as a critical sensor of both pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) . Activation of TLR4 triggers pro-inflammatory signaling cascades, leading to the release of cytokines, chemokines, and reactive oxygen species. Mounting evidence implicates TLR4 in the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS) . Chronic TLR4 activation contributes to sustained neuroinflammation, synaptic dysfunction, and neuronal loss. Moreover, TLR4 may mediate the brain’s response to misfolded proteins like amyloid-β and α-synuclein, linking innate immunity to proteinopathy-driven neurodegeneration. Given its dual role in host defense and neuroinflammatory signaling, TLR4 is a promising therapeutic target for modulating immune responses and mitigating neurodegenerative progression.
Description
Rabbit Anti-Human TLR4 Polyclonal
CAS Number
9007-83-4
Specifications
Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Product Name Alternative
ARMD10, CD284, TLR 4, TOLL, Toll like receptor 4
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
7099
Swiss Prot
O00206
Accession Number
NP_612564.1
Cellular Locus
Membrane
Host
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Target
TLR4
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal
Applications
WB | IHC | ICC/IF | FCM | FACS
Validated Applications
WB, IHC, ICC/IF, FCM, FACS
Field of Research
Immunology | Innate Immunity | Macrophage | Immunology | Immunoglobulins | Immunoglobulin Receptors | Cardiovascular System | Atherosclerosis | Vascular Inflammation | Inflammatory Mediators | Immunology | Adaptive Immunity | Regulatory T Cells | Immunology | Innate Immunity | TLR Signaling
Purification
Protein A Purified
Detection Range
2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:500), IHC (1:50) ; optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518 (1-2) : 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388 (6640) : 394-397.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Specificity
Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag) .
Species
Human | Mouse
Location
Membrane
Immunogen Species
Human

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