TLR4 Antibody

• Background:

  Toll-like receptor 4 (TLR4) is a transmembrane pattern recognition receptor and a central component of the innate immune system. Structurally, TLR4 is defined by extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain, which mediates downstream signaling through adaptor proteins such as MyD88 and TRIF. Among the ten known human TLRs (TLR1–TLR10), TLR4 is one of the most extensively studied, particularly for its role in recognizing lipopolysaccharide (LPS) from Gram-negative bacteria. In the central nervous system, TLR4 is expressed on microglia, astrocytes, and neurons, where it serves as a critical sensor of both pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) . Activation of TLR4 triggers pro-inflammatory signaling cascades, leading to the release of cytokines, chemokines, and reactive oxygen species. Mounting evidence implicates TLR4 in the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS) . Chronic TLR4 activation contributes to sustained neuroinflammation, synaptic dysfunction, and neuronal loss. Moreover, TLR4 may mediate the brain’s response to misfolded proteins like amyloid-β and α-synuclein, linking innate immunity to proteinopathy-driven neurodegeneration. Given its dual role in host defense and neuroinflammatory signaling, TLR4 is a promising therapeutic target for modulating immune responses and mitigating neurodegenerative progression.

• Description:

  Rabbit Anti-Human TLR4 Polyclonal

• Specifications:

  Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

• Product Name Alternative:

  ARMD10, CD284, TLR 4, TOLL, Toll like receptor 4

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  7099

• Swiss Prot:

  O00206

• Accession Number:

  NP_612564.1

• Cellular Locus:

  Membrane

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse

• Immunogen:

  Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

• Target:

  TLR4

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | IHC | ICC/IF | FCM | FACS

• Validated Applications:

  WB, IHC, ICC/IF, FCM, FACS

• Field of Research:

  Immunology | Innate Immunity | Macrophage | Immunology | Immunoglobulins | Immunoglobulin Receptors | Cardiovascular System | Atherosclerosis | Vascular Inflammation | Inflammatory Mediators | Immunology | Adaptive Immunity | Regulatory T Cells | Immunology | Innate Immunity | TLR Signaling

• Purification:

  Protein A Purified

• Detection Range:

  2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:500), IHC (1:50) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518 (1-2) : 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388 (6640) : 394-397.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag) .

• Species:

  Human | Mouse

• CAS Number:

  9007-83-4

• Location:

  Membrane

• Immunogen Species:

  Human