Phosphothreonine Antibody

• Background:

  Phosphothreonine is a phosphorylated derivative of threonine, catalyzed by serine/threonine kinases. It serves as a regulatory switch in numerous signaling pathways that govern neuronal survival, synaptic function, and stress responses. In the brain, phosphothreonine modifications influence the activity of key proteins such as tau, MAPKs, and transcription factors. Dysregulated threonine phosphorylation is associated with protein misfolding, impaired axonal transport, and neuroinflammation—hallmarks of neurodegenerative diseases. Notably, abnormal phosphorylation of tau at threonine residues contributes to its aggregation and toxicity in Alzheimer’s disease. As a modifiable and site-specific event, phosphothreonine is a valuable marker for disease progression and a potential target for kinase-based therapies in neurodegeneration.

• Description:

  Rabbit Anti-Phosphothreonine Polyclonal

• Specifications:

  Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

• Product Name Alternative:

  O-phospho-L-threonine, Phospho-threonine

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Host:

  Rabbit

• Species Reactivity:

  Species Independent

• Immunogen:

  Phosphothreonine conjugated to KLH

• Target:

  Phosphothreonine

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | ICC/IF | IP | ELISA

• Validated Applications:

  WB, ICC/IF, IP, ELISA

• Field of Research:

  Cell Signaling | Post-translational Modifications | Phosphorylation

• Purification:

  Peptide Affinity Purified

• Detection Range:

  2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

• Concentration:

  0.25 mg/ml

• Dilution:

  WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.4

• Buffer:

  PBS, 50% glycerol, 0.01% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9 (16) : 2034-2041.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.

• Species:

  Species Independent

• CAS Number:

  9007-83-4