Phosphothreonine Antibody

• Background:

  Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity

• Synonyms:

  Phosphoser/thr mAb

• Modification Site:

  PThr

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  Anti-Phosphothreonine

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  Phosphothreonine synthetic peptides coupled to carrier protein.

• Applications:

  WB, IP

• Purification:

  Antigen Affinity purification

• Dilution:

  WB (1:300-5000), IP (1-2ug)

• Buffer:

  PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

• Modification:

  Phosphorylation

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• Specificity:

  This antibody was cross-adsorbed to unphosphorylated peptide then Affinity purification using phospho-threonine peptide (without carrier) . The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA.