HSP90 Antibody

HSP90 is a highly conserved and abundantly expressed molecular chaperone that plays a central role in maintaining protein homeostasis, particularly in the nervous system. Present in all eukaryotic cells, HSP90 exists in two major cytosolic isoforms—HSP90α and HSP90β—which share 85% sequence identity but differ in oligomeric state and regulatory function. Despite its classification as a heat shock protein, HSP90 is constitutively expressed at high levels, comprising up to 2% of total cytosolic protein in unstressed cells. It is essential for the folding, maturation, and stabilization of a wide range of client proteins, many of which are involved in neuronal signaling, synaptic plasticity, and stress response. These include kinases (e.g., c-Raf), transcription factors (e.g., p53), and steroid hormone receptors. In neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s, HSP90 is implicated in both protective and pathological processes. It stabilizes misfolded proteins and prevents aggregation, but can also shield aberrant proteins from degradation, contributing to disease progression. HSP90’s interaction with co-chaperones like Cdc37 and p23 forms complexes that regulate the fate of client proteins, making it a key node in proteostasis networks. Pharmacological inhibition of HSP90—using compounds like geldanamycin—has shown promise in modulating protein quality control pathways and reducing toxic protein accumulation in neurodegenerative models.

Rabbit Anti-Human HSP90 Polyclonal

9007-83-4

Detects ~90kDa.

HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

12352203

Non-hazardous

Non-hazardous

3326

P08238

NP_031381.2

Cytoplasm | Melanosome

Full length Human Hsp90 protein

HSP90

Unconjugated

Polyclonal

WB | IHC | ICC/IF | IP | ELISA

Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers

Protein A Purified

A 1:500 dilution of SPC-104 was sufficient for detection of 0.2 mg of purified HSP90 by ECL immunoblot analysis.

1 mg/ml

WB (1:500), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

0.012

PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev 18: 306–360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med 217: 420–434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.

Blue Ice or 4ºC

-20ºC

Detects ~90kDa.

Human | Mouse | Rat

Cytoplasm | Melanosome