HSP90 beta Protein

• Background:

  HSP90 beta is the constitutively expressed isoform of the HSP90 family and is essential for the maintenance of cellular protein networks. In the nervous system, HSP90 beta supports the folding and maturation of proteins involved in synaptic transmission, cytoskeletal dynamics, and neuronal survival. Emerging evidence links HSP90 beta to neurodegenerative disease mechanisms, particularly through its regulation of tau, α-synuclein, and other aggregation-prone proteins. Alterations in HSP90 beta expression or function can disrupt neuronal signaling pathways and exacerbate protein misfolding, contributing to the pathogenesis of disorders such as Alzheimer’s and Parkinson’s disease. HSP90 beta interacts with a broad array of co-chaperones and client proteins, forming cytosolic complexes that buffer cells against proteotoxic stress. Its role in modulating the stability and degradation of neurotoxic proteins makes it a potential therapeutic target for restoring proteostasis and mitigating neurodegeneration.

• Description:

  Human Recombinant HSP90 beta Full Length Protein

• Product Name Alternative:

  FLJ26984, HSP84, HSP90, HSP90B, HSPC2, HSPCB

• UNSPSC:

  12352202

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  3326

• Swiss Prot:

  P08238

• Accession Number:

  NP_031381.2

• Cellular Locus:

  Cytoplasm | Melanosome

• Expression System:

  Baculovirus/Sf9

• Host:

  Baculovirus/Sf9 cells

• Origin Species:

  Human

• Target:

  HSP90 beta

• Conjugation:

  No tag

• Nature:

  Recombinant

• Sequence:

  MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD

• Applications:

  WB | SDS-PAGE | Functional Assay | ELISA | Co-IP | Surface Plasmon Resonance (SPR)

• Field of Research:

  Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers

• Purification Method:

  Affinity Purified | Low Endotoxin

• Purification:

  Affinity Purified | Low Endotoxin

• Limit Of Detection:

  This product has been certified >90% pure using SDS-PAGE analysis.

• Concentration:

  Lot/batch specific. See included datasheet.

• Purity:

  >85%

• Activity:

  ATPase active

• Weight:

  0.05

• Length:

  Full Length

• Buffer:

  20mM Tris, pH7.5, 175 mM NaCl, 0.1 mM EDTA, 10% glycerol, 1 mM DTT

• Molecular Weight:

  ~90 kDa

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

• References & Citations:

  1. Arlander S.J.H., et al. (2003) J Biol Chem. 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem. 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med. 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med. 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev. 18: 306–360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med. 217: 420–434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA. 91: 8324– 8328.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Protein Length:

  Full Length

• Background Reference 01:

  1. Arlander S.J.H., et al. (2003) J Biol Chem. 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem. 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med. 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med. 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev. 18: 306–360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med. 217: 420–434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA. 91: 8324– 8328.

• Location:

  Cytoplasm | Melanosome

• AA Sequence:

  MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD

• Immunogen Species:

  Human