PP5 Antibody

• Background:

  Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 or PPT is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phosphorylase and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1,2).

• Description:

  Mouse Anti-Human PP5 Monoclonal IgG1

• Specifications:

  Detects ~58kDa.

• Product Name Alternative:

  PPP5 Antibody, PPT Antibody, Protein phosphatase T Antibody, Serine/threonine protein phosphatase 5 Antibody

• Synonyms:

  PP5 Antibody, Clone 12F7

• UNSPSC:

  12352203

• Gene ID:

  5536

• Swiss Prot:

  P53041

• Accession Number:

  NP_006238.1

• Cellular Locus:

  Nucleus | Cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Full length human PP5 protein

• Target:

  PP5

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  12F7

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, ICC/IF

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml of SMC-244 was sufficient for detection of PP5 in 15 µg of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Butzow R., et al. (1988) J Lab Clin Med. 111(2): 249-256.

• CAS Number:

  9007-83-4