Trap1 Antibody

• Background:

  The 90 kDa heat shock protein (HSP90) family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).

• Description:

  Mouse Anti-Human Trap1 Monoclonal IgG1 Kappa

• Specifications:

  Detects ~75kDa.

• Product Name Alternative:

  Heat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody

• Synonyms:

  TRAP1 Antibody, Clone 3H4-2H6

• UNSPSC:

  12352203

• Gene ID:

  10131

• Swiss Prot:

  Q12931

• Accession Number:

  NP_057376.2

• Cellular Locus:

  Mitochondrion

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Purified recombinant Human TRAP2

• Target:

  TRAP1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  3H4-2H6

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, ICC/IF

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312. 2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46. 3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.

• CAS Number:

  9007-83-4