DNMT1 Antibody

• Background:

  Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

• Description:

  Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa

• Specifications:

  Detects ~180kDa. It will cross-react with mouse DNMT1.

• Product Name Alternative:

  DNA Mtase Antibody, DNMT Antibody, MCMT Antibody, DNA methyltransferase 1 Antibody, AIM Antibody, CXXC9 Antibody, DNMT Antibody, DNA (cytosine-5)-methyltransferase 1 Antibody, CXXC-type zinc finger protein 9 Antibody, DNA methyltransferase HsaI Antibody

• Synonyms:

  DNMT1 Antibody, Clone 4G11-C7

• UNSPSC:

  12352203

• Gene ID:

  1786

• Swiss Prot:

  P26358

• Accession Number:

  NP_001370

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Fish, Zebrafish

• Immunogen:

  Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1

• Target:

  DNMT1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  4G11-C7

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, IHC, IP, ChIP

• Purification:

  Protein G Purified

• Limit Of Detection:

  2 µg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.

• CAS Number:

  9007-83-4