DNMT1 Antibody

CAT: 0400-SMC-201DSize: 100 µgDry Ice: NoHazardous: No
CAT#:0400-SMC-201DSize:100 µg
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Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Description
Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa
CAS Number
9007-83-4
Specifications
Detects ~180kDa. It will cross-react with mouse DNMT1.
Product Name Alternative
DNA Mtase Antibody, DNMT Antibody, MCMT Antibody, DNA methyltransferase 1 Antibody, AIM Antibody, CXXC9 Antibody, DNMT Antibody, DNA (cytosine-5)-methyltransferase 1 Antibody, CXXC-type zinc finger protein 9 Antibody, DNA methyltransferase HsaI Antibody
Synonyms
DNMT1 Antibody, Clone 4G11-C7
UNSPSC
12352203
Gene ID
1786
Swiss Prot
P26358
Accession Number
NP_001370
Cellular Locus
Nucleus
Host
Mouse
Species Reactivity
Human, Mouse, Fish, Zebrafish
Immunogen
Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
Target
DNMT1
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
4G11-C7
Conjugation
Unconjugated
Validated Applications
WB, IHC, IP, ChIP
Purification
Protein G Purified
Limit Of Detection
2 µg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
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