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Urine Creatinine Detection Kit

CAT: 0400-SKT-200-192Size: 2x 96 WellDry Ice: NoHazardous: No
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CAT#:0400-SKT-200-192Size:2x 96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Creatinine is a metabolic byproduct of phosphocreatine, a high-energy phosphate reservoir used in ATP regeneration. It is produced at a relatively constant rate and excreted by the kidneys, making it a reliable internal standard for normalizing biomarker concentrations in urine. In neuroscience research, urinary creatinine is commonly used to adjust for variations in hydration and renal function when measuring oxidative stress markers such as 8-OHdG or nitrotyrosine. This normalization is essential for accurate comparisons across individuals and time points. Although not a direct marker of neurodegeneration, altered creatinine levels may reflect systemic conditions—such as diabetes or cardiovascular disease—that influence brain health. Its role in standardizing biomarker data makes creatinine an indispensable tool in neurobiological and clinical studies.
Description
Quantitative colorimetric detection of creatinine in urine samples
Overview
The Urine Creatinine Detection kit is designed to quantitatively measure creatinine present in urine samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine (7,8) .
Product Name Alternative
Methylguanidoacetic acid, N-Carbamimidoyl-N-methylglycine
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Species Reactivity
Human, Monkey, Dog, Rat
Target
Urine Creatinine Detection Kit
Type
Detection Kits
Applications
Detection kit used to quantitatively measure creatinine in samples.
Field of Research
Cell Signaling | Cardiovascular System
Detection Method
Colorimetric Assay
Assay Type
Direct Quantitative Assay
Assay Protocol
The Urine Creatinine Detection kit is designed to quantitatively measure creatinine present in urine samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine(7,8).
Sample Type
Urine
Sample Volume
88 samples in duplicate
Detection Range
0.3125- 20 mg/dl
Precision
Intra Assay Precision: Three human urine samples were diluted 1:20 with deionized water and run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human urine samples were diluted 1:20 with deionized water and run in duplicates in 20 assays run over five days by three operators. The mean and precision of the calculated creatinine concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV
Sensitivity
0.032 mg/dl
Weight
300
Components
SKC-200A | SKC-200B | SKC-200C | SKC-200D
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
Shipping Conditions
Blue Ice
Storage Conditions
4ºC
Background Reference 01
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
Species
Human | Monkey | Dog | Rat
Quantity
2 Plates | 1 ml | 20 ml | 2 each
Platform
Microplate

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