Blood Urea Nitrogen (BUN) Detection Kit

• Background:

  Urea is a nitrogenous waste product formed in the liver during protein metabolism and excreted by the kidneys. Blood Urea Nitrogen (BUN) levels are commonly used to assess renal function, with normal adult values ranging from 7–21 mg/dL. Elevated BUN (azotemia) may indicate kidney dysfunction, dehydration, or systemic illness. In neuroscience, impaired renal clearance of urea and other metabolites can contribute to cognitive dysfunction and neuroinflammation. Uremic toxins have been implicated in the development of encephalopathy and may exacerbate neurodegenerative conditions. Non-invasive sampling of urea in urine or saliva, alongside serum creatinine, provides a valuable tool for monitoring systemic health in neurological research.

• Description:

  Quantitative colorimetric measurement of urea nitrogen

• Product Name Alternative:

  BUN, Blood urea nitrogen

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Species Reactivity:

  Species Independent

• Target:

  Blood Urea Nitrogen (BUN) Detection Kit

• Type:

  Detection Kits

• Applications:

  Colorimetric assay used to quantitatively measure urea nitrogen in a variety of samples.

• Field of Research:

  Cell Signaling | Cardiovascular System

• Detection Method:

  Colorimetric Assay

• Assay Type:

  Direct Quantitative Assay

• Assay Protocol:

  The Blood Urea Nitrogen (BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14.

• Sample Type:

  Serum | Plasma | Urine | Saliva | Tissue Culture Media

• Sample Volume:

  88 samples in duplicate

• Detection Range:

  0.156 - 10 mg/dl

• Precision:

  Intra-Assay Precision (Within Run Precision) To determine Intra-Assay Precision, three samples of known concentration were assayed at different times, with different operators. The intra-assay coefficient of variation of the BUN within assays was determined to be <10%. Inter-Assay Precision (Between Run Precision) To determine Inter-Assay Precision, three samples of known concentration were assayed at different times, with different operators. The inter-assay coefficient of variation of the BUN between assays was determined to be <20%.

• Sensitivity:

  0.042 mg/dl

• Weight:

  300

• Components:

  SKC-213A | SKC-213B | SKC-213C | SKC-213D | SKC-213E

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Laboratory reference values. Urea nitrogen (BUN). Rochester, Minn.: Mayo Foundation for Medical Education and Research; Nov. 2010. 2. Waiker, SS and JV Bonventre. (2008) Nephron Clin. Pract. 109:c192-c197. 3. Al Mofleh, IA. World J. Gastroent. (2008) Congestive heart failure. 14(5):675-684. 4. Iglesiase, J. et al. (2006) Nephrol. Dial. Transplant. 21:3458-3465. 5. Mayo Clinic. “Blood urea nitrogen (BUN) tests.” http://www.mayoclinic.com/health/blood-urea-nitrogen/MY00373/DSECTION=results 6. Lum, G and S Leal-Khouri. (1989) Clin. Chem. 35(4):639-640. 7. Akai, T, et al. (1983) Clin. Chem. 1983. 29(10):1825-1827.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4ºC and -20ºC

• Background Reference 01:

  1. Laboratory reference values. Urea nitrogen (BUN) . Rochester, Minn.: Mayo Foundation for Medical Education and Research; Nov. 2010. 2. Waiker, SS and JV Bonventre. (2008) Nephron Clin. Pract. 109:c192-c197. 3. Al Mofleh, IA. World J. Gastroent. (2008) Congestive heart failure. 14 (5) :675-684. 4. Iglesiase, J. et al. (2006) Nephrol. Dial. Transplant. 21:3458-3465. 5. Mayo Clinic. “Blood urea nitrogen (BUN) tests.” http://www.mayoclinic.com/health/blood-urea-nitrogen/MY00373/DSECTION=results 6. Lum, G and S Leal-Khouri. (1989) Clin. Chem. 35 (4) :639-640. 7. Akai, T, et al. (1983) Clin. Chem. 1983. 29 (10) :1825-1827.

• Species:

  Species Independent

• Incubation Time:

  30 Minutes

• Overview:

  The Blood Urea Nitrogen (BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14.

• Quantity:

  2 Plates | 250 µl | 15 ml | 15 ml | 2 vials

• Platform:

  Microplate