Corticosterone EIA Kit

Corticosterone (C₂₁H₃₀O₄) is a glucocorticoid hormone secreted by the adrenal cortex in response to ACTH stimulation. It is the primary stress hormone in rodents and a precursor to aldosterone. In neuroscience research, corticosterone is widely used to model chronic stress and its effects on brain function. Elevated corticosterone levels have been shown to impair memory retrieval, alter sleep-wake cycles, and exacerbate neurodegenerative processes. In Parkinson’s disease models, chronic corticosterone exposure increases alpha-synuclein phosphorylation and aggregation, particularly in the hypothalamus, and contributes to dopaminergic neuron loss. These findings highlight corticosterone’s role in stress-induced neurotoxicity and its relevance in studying the pathophysiology of neurodegenerative and psychiatric disorders.

Colorimetric detection of corticosterone

The Corticosterone EIA kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

7732-18-5

(11β) -11,21-dihydroxypregn-4-ene-3,20-dione

12352203

Non-hazardous

Non-hazardous

Corticosterone EIA Kit

EIA Kits

EIA kit used to measure the corticosterone present in samples.

Cell Signaling | Cancer | Oxidative Stress | Neuroscience

Colorimetric Assay

Sandwich EIA (Enzyme Immunoassay)

The Corticosterone EIA kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Serum | EDTA Plasma | Heparin Plasma | Urine | Tissue Culture Media | Dried Fecal Samples

39 samples in duplicate

78.125 - 10,000 pg/ml

Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Corticosterone concentrations were: Sample 1- 2460.6 pg/mL, 6.3% CV Sample 2- 601.5 pg/mL, 6.5% CV Sample 3- 371.6 pg/mL, 3.1% CV Sample 4- 259.0 pg/mL, 4.8% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in fourteen assays run over multiple days by four operators. The mean and precision of the calculated Corticosterone concentrations were: Sample 1- 2618.3 pg/mL, 7.5% CV Sample 2- 630.1 pg/mL, 6.4% CV Sample 3- 267.9 pg/mL, 9.9% CV

18.6 pg/ml

300

SKC-205A | SKC-205B | SKC-205C | SKC-205D | SKC-205E | SKC-205F | SKC-205G | SKC-205H | SKC-205I | SKC-205J

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Hupé, JM, et al. Nature, 1998; 394: 784-787. 2. Kitaysky AS, Kitaiskaia EV, Wingfield JC, Piatt JF. J. Comp. Physiol, 2001; 171: 701-709. 3. Thellin O, Noel G, Khuana S, Ogle CK and Horseman ND. Shock, 2001; 16(5): 393-397. 4. Krame, KM. and Sothern RB. Chronobiol. Int., 2001; 18(6): 933-945. 5. Vazquez-Palacios G, et al. Pharmacol. Biochem Behavior, 2001: 70(2-3): 305-310.

Blue Ice

4ºC and -20ºC

1. Hupé, J. M., James, A. C., Payne, B. R., Lomber, S. G., Girard, P., & Bullier, J. (1998) . Cortical feedback improves discrimination between figure and background by V1, V2, and V3 neurons. Nature, 394 (6695), 784–787. DOI: 10.1038/29555 2. Kitaysky, A. S., Kitaiskaia, E. V., Wingfield, J. C., & Piatt, J. F. (2001) . Dietary restrictions cause chronic elevation of corticosterone and enhance stress response in red-legged kittiwake chicks. Journal of Comparative Physiology B, 171 (8), 701–709. DOI: 10.1007/s003600100230 3. Thellin, O., Noel, G., Khuana, S., Ogle, C. K., & Horseman, N. D. (2001) . Stress hormone secretion and gut signal transducer (STAT) proteins after burn injury in rats. Shock, 16 (5), 393–397. DOI: 10.1097/00024382-200116050-00001 4. Kramer, K. M., & Sothern, R. B. (2001) . Circadian characteristics of corticosterone secretion in red-backed voles (Clethrionomys gapperi) . Chronobiology International, 18 (6), 933–945. DOI: 10.1081/CBI-100107542 5. Vazquez-Palacios, G., Retana-Márquez, S., Bonilla-Jaime, H., & Velázquez-Moctezuma, J. (2001) . Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacology, Biochemistry, and Behavior, 70 (2–3), 305–310. DOI: 10.1016/S0091-3057 (01) 00629-8 6. Bimpos, N. M., Xie, Y., Gomez-Isla, T., Domenger, D., & Hyman, B. T. (2024) . Alpha-synuclein-induced stress sensitivity renders the Parkinson’s disease brain susceptible to neurodegeneration. Acta Neuropathologica Communications, 12, 100. DOI: 10.1186/s40478-024-01797-w. 7. Burtscher, J., Copin, J. C., Rodrigues, J., & Pernet, N. (2019) . Chronic corticosterone aggravates behavioral and neuronal symptomatology in a mouse model of alpha-synuclein pathology. Neurobiology of Aging, 83, 11–20. DOI: 10.1016/j.neurobiolaging.2019.08.011

Species Independent

1 Each | 125 µl | 3 ml | 3 ml | 28 ml | 1 ml | 30 ml | 11 ml | 5 ml | 1 Each