Creatinine Serum Detection Kit

• Background:

  Creatinine is a metabolic byproduct of phosphocreatine, a high-energy phosphate reservoir used in ATP production. It is produced at a relatively constant rate and excreted by the kidneys without reabsorption, making it a reliable marker of renal function. In neuroscience, creatinine is widely used to normalize urinary biomarker levels—such as 8-OHdG or nitrotyrosine—ensuring accurate comparisons across individuals and time points. Its stability and low intra-individual variability (<15%) make it ideal for longitudinal studies. Altered creatinine levels may also reflect systemic conditions like diabetes or cardiovascular disease, which are known to impact brain health and increase the risk of neurodegeneration.

• Description:

  Colorimetric measurement of creatinine

• Product Name Alternative:

  Methylguanidoacetic acid, N-Carbamimidoyl-N-methylglycine

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Species Reactivity:

  Human, Mouse, Rabbit, Rat, Sheep

• Target:

  Creatinine Serum Detection Kit

• Type:

  Detection Kits

• Applications:

  Colorimetric assay used to measure creatinine in samples.

• Field of Research:

  Cell Signaling | Cardiovascular System

• Detection Method:

  Colorimetric Assay

• Assay Type:

  Direct Quantitative Assay

• Assay Protocol:

  The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read in a microtiter plate reader capable of measuring 490nm wavelength. For a total of 30 minutes, the optical density is read at 1 minute intervals. The concentration of creatinine is calculated using the Kinetic Mode software and samples are compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum (8).

• Sample Type:

  Serum | Plasma

• Sample Volume:

  91 samples in duplicate

• Detection Range:

  0.5 - 4 mg/dl

• Precision:

  Intra Assay Precision: Four human serum samples were run in triplicate on a same-day assay determined that the mean and precision of the calculated concentrations were: Sample 1- 0.75 mg/dL, 5% CV Sample 2- 0.78 mg/dL, 6% CV Sample 3- 0.6 mg/dL, 4% CV Sample 4- 0.76 mg/dL, 6% CV Inter Assay Precision: Four human serum samples were run in triplicate on a same-day assay determined that the total range of 4-6% represents the variation between different assays.

• Sensitivity:

  0.085 mg/dl

• Weight:

  300

• Components:

  SKC-217A | SKC-217B | SKC-217C | SKC-217D

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4ºC

• Background Reference 01:

  1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.

• Species:

  Human | Mouse | Rabbit | Rat | Sheep

• Overview:

  The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read in a microtiter plate reader capable of measuring 490nm wavelength. For a total of 30 minutes, the optical density is read at 1 minute intervals. The concentration of creatinine is calculated using the Kinetic Mode software and samples are compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum (8) .

• Quantity:

  2 Plates | 100 µl | 6 ml | 20 ml

• Platform:

  Microplate