Creatinine Serum Detection Kit

CAT: 0400-SKT-217-192Size: 2x 96 WellDry Ice: NoHazardous: No
CAT#:0400-SKT-217-192Size:2x 96 Well
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Background
Creatinine is a metabolic byproduct of phosphocreatine, a high-energy phosphate reservoir used in ATP production. It is produced at a relatively constant rate and excreted by the kidneys without reabsorption, making it a reliable marker of renal function. In neuroscience, creatinine is widely used to normalize urinary biomarker levels—such as 8-OHdG or nitrotyrosine—ensuring accurate comparisons across individuals and time points. Its stability and low intra-individual variability (<15%) make it ideal for longitudinal studies. Altered creatinine levels may also reflect systemic conditions like diabetes or cardiovascular disease, which are known to impact brain health and increase the risk of neurodegeneration.
Description
Colorimetric measurement of creatinine
Overview
The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read in a microtiter plate reader capable of measuring 490nm wavelength. For a total of 30 minutes, the optical density is read at 1 minute intervals. The concentration of creatinine is calculated using the Kinetic Mode software and samples are compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum (8) .
Product Name Alternative
Methylguanidoacetic acid, N-Carbamimidoyl-N-methylglycine
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Species Reactivity
Human, Mouse, Rabbit, Rat, Sheep
Target
Creatinine Serum Detection Kit
Type
Detection Kits
Applications
Colorimetric assay used to measure creatinine in samples.
Field of Research
Cell Signaling | Cardiovascular System
Detection Method
Colorimetric Assay
Assay Type
Direct Quantitative Assay
Assay Protocol
The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read in a microtiter plate reader capable of measuring 490nm wavelength. For a total of 30 minutes, the optical density is read at 1 minute intervals. The concentration of creatinine is calculated using the Kinetic Mode software and samples are compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum (8).
Sample Type
Serum | Plasma
Sample Volume
91 samples in duplicate
Detection Range
0.5 - 4 mg/dl
Precision
Intra Assay Precision: Four human serum samples were run in triplicate on a same-day assay determined that the mean and precision of the calculated concentrations were: Sample 1- 0.75 mg/dL, 5% CV Sample 2- 0.78 mg/dL, 6% CV Sample 3- 0.6 mg/dL, 4% CV Sample 4- 0.76 mg/dL, 6% CV Inter Assay Precision: Four human serum samples were run in triplicate on a same-day assay determined that the total range of 4-6% represents the variation between different assays.
Sensitivity
0.085 mg/dl
Weight
300
Components
SKC-217A | SKC-217B | SKC-217C | SKC-217D
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
Shipping Conditions
Blue Ice
Storage Conditions
4ºC
Background Reference 01
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
Species
Human | Mouse | Rabbit | Rat | Sheep
Quantity
2 Plates | 100 µl | 6 ml | 20 ml
Platform
Microplate

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