TFEB rabbit pAb

• Background:

  Function:Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer., similarity:Belongs to the MiT/TFE family., similarity:Contains 1 basic helix-loop-helix (bHLH) domain., subunit:Efficient DNA binding requires dimerization with another bHLH protein.

• Description:

  Function: Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer. similarity: Belongs to the MiT/TFE family. similarity: Contains 1 basic helix-loop-helix (bHLH) domain. subunit: Efficient DNA binding requires dimerization with another bHLH protein.

• UniProt:

  P19484

• Swiss Prot:

  P19484

• Reactivity:

  Human; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human TFEB. AA range:10-59

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

• Molecular Weight:

  52kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  52kD

• Fragment:

  IgG

• Subcellular Location:

  Cytoplasm, cytosol . Lysosome membrane . Nucleus . Mainly present in the cytoplasm (PubMed:23434374, PubMed:33691586) . Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:21617040, PubMed:22576015, PubMed:23434374) . The translocation to the nucleus is regulated by ATP13A2 (PubMed:23434374, PubMed:27278822) . Colocalizes with mTORC1 on the lysosomal membrane: when nutrients are present, phosphorylation by MTOR prevents nuclear translocation and activity (PubMed:22343943, PubMed:22692423) . Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22343943, PubMed:22692423) . Exported from the nucleus in response to nutrient availability (PubMed:30120233) . In macro

• Other Product Names:

  TFEB; BHLHE35; Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35

• Gene ID (Human):

  7942