TFEB rabbit pAb

• Background :

  Function:Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer., similarity:Belongs to the MiT/TFE family., similarity:Contains 1 basic helix-loop-helix (bHLH) domain., subunit:Efficient DNA binding requires dimerization with another bHLH protein.

• Description :

  Function: Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer. similarity: Belongs to the MiT/TFE family. similarity: Contains 1 basic helix-loop-helix (bHLH) domain. subunit: Efficient DNA binding requires dimerization with another bHLH protein.

• UniProt :

  P19484

• Swiss Prot :

  P19484

• Reactivity :

  Human; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human TFEB. AA range:10-59

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

• Molecular Weight :

  52kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  52kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm, cytosol . Lysosome membrane . Nucleus . Mainly present in the cytoplasm (PubMed:23434374, PubMed:33691586) . Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:21617040, PubMed:22576015, PubMed:23434374) . The translocation to the nucleus is regulated by ATP13A2 (PubMed:23434374, PubMed:27278822) . Colocalizes with mTORC1 on the lysosomal membrane: when nutrients are present, phosphorylation by MTOR prevents nuclear translocation and activity (PubMed:22343943, PubMed:22692423) . Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22343943, PubMed:22692423) . Exported from the nucleus in response to nutrient availability (PubMed:30120233) . In macro

• Other Product Names :

  TFEB; BHLHE35; Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35

• Gene ID (Human) :

  7942