Mouse anti Integrin beta 4 (CD104)

Mouse anti Integrin beta 4 (CD104)

• Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. CD104 is a 205kD type I transmembrane glycoprotein, also known as beta 4 integrin. CD104 assoaciates with CD49f (integrin alpha 6) forming a heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation.
• CAS Number: 9007-83-4
• UniProt: P16144
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG2a
• Clone: 58XB4
• Type: Primary Antibodies
• Source: 58XB4 is a monoclonal antibody obtained by immunization of Balb/c mice with Integrin β4. The immunogen used to raise this antibody was the full length CD104 protein.
• Applications: ELISA, Flow Cytometry, IF, IHC (frozen), IP, WB, ICC
• Field of Research: Cell adhesion, Immunology, CD Markers
• Assay Principle: 58XB4 is useful for Enzyme Linked Immuno Sorbent Assays (ELISA), Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western blotting, immunocytochemistry of paraformaldehyde fixed cells and Immubohistochemistry on frozen sections. For protocols see reference 1. Optimal antibody dilution should be determined by titration.
• Form: Each vial contains 100 µL 1 mg/mL purified monoclonal antibody in PBS containing 0,09% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• References & Citations: 1. Dirk Geerts, Lionel Fontao, Mirjam G. Nievers, Roel Q.J. Schaapveld, Patricia E. Purkis, Grant N. Wheeler, E. Birgitte Lane, Irene M. Leigh, and Arnoud Sonnenberg. J Cell Biol. 1999 October 18; 147(2): 417–434.
• Storage Conditions: The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product