BID (Cleaved-Arg71) rabbit pAb

• Background:

  Domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family., function:The major proteolytic product p15 BID allows the release of cytochrome c (By similarity) . Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., PTM:TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products., subcellular location:A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively., subcellular location:Associated with the mitochondrial membrane., subcellular location:Translocates to mitochondria as an integral membrane protein., subcellular location:When uncleaved, it is predominantly cytoplasmic., subunit:Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2., tissue specificity:Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) . Isoform 3 is expressed in lung, pancreas and spleen (at protein level) . Isoform 4 is expressed in lung and pancreas (at protein level) .

• Description:

  Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. function: The major proteolytic product p15 BID allows the release of Cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. subcellular location: A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. subcellular location: Associated with the mitochondrial membrane. subcellular location: Translocates to mitochondria as an integral membrane protein. subcellular location: When uncleaved, it is predominantly cytoplasmic. subunit: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2. tissue specificity: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

• UniProt:

  P55957

• Swiss Prot:

  P55957

• Reactivity:

  Human; Mouse

• Immunogen:

  Synthesized peptide derived from human BID (Cleaved-Arg71)

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  WB 1:1000-2000 ELISA 1:5000-20000

• Molecular Weight:

  8 22kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  8 22kD

• Fragment:

  IgG

• Subcellular Location:

  Cytoplasm . Mitochondrion membrane . Mitochondrion outer membrane . When uncleaved, it is predominantly cytoplasmic. .; [BH3-interacting domain death agonist p15]: Mitochondrion membrane . Translocates to mitochondria as an integral membrane protein. .; [BH3-interacting domain death agonist p13]: Mitochondrion membrane . Associated with the mitochondrial membrane. .; [Isoform 1]: Cytoplasm .; [Isoform 3]: Cytoplasm .; [Isoform 2]: Mitochondrion membrane . A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. .

• Other Product Names:

  BH3-interacting domain death agonist (p22 BID; BID) [Cleaved into: BH3-interacting domain death agonist p15 (p15 BID) ; BH3-interacting domain death agonist p13 (p13 BID) ; BH3-interacting domain death agonist p11 (p11 BID) ]

• Gene ID (Human):

  637