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Recombinant E.coli NusA protein

CAT: 0112-NUS0801-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-NUS0801-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NusA is a key component in both Prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes. Recombinant NusA was expressed in E. coli and purified by using conventional chromatography techniques
Product Name Alternative
Transcription termination/antitermination L factor, ECK3158, JW3158, Transcription termination/antitermination L factor, NusA, Transcription termination/antitermination L factor
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
54 kDa (495aa) confirmed by MALDI-TOF
Additionnal Information
NusA, NUS0801-10 µg, NUS0801-20 µg, NUS0801-50 µg, NUS0801-100 µg, NUS0801-250 µg, NUS0801-500 µg, NUS0801-1 mg, NUS0801-10, NUS0801-20, NUS0801-50, NUS0801-100, NUS0801-250, NUS0801-500, NUS0801-1
References & Citations
Carlomagno MS., et al. (2003) Gene. 308:115-28.; ; Gusarov I., . (2001) Cell. 107 (4) :437-49.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Fusion Tag & Detection
NCBI Accession Number
AAC76203
Uniprot Accession Number
P0AFF6
Species
E.coli
AA Sequence
MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA

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