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Recombinant E.coli udp protein

CAT: 0112-ATGP0661-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0661-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Uridine phosphorypase (uDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, urdPase, uPH, and uPase. Recombinant E. coli uDP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.
Product Name Alternative
Uridine phosphorylase, UrdPase, Upase
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 20,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.
Molecular Weight
29.3 kDa (273aa) confirmed by MALDI-TOF
Additionnal Information
UDP, uridine phosphorylase, urdPase, upase, uridine phosphorylase, ATGP0661-10 µg, ATGP0661-20 µg, ATGP0661-50 µg, ATGP0661-100 µg, ATGP0661-250 µg, ATGP0661-500 µg, ATGP0661-1 mg, ATGP0661-10, ATGP0661-20, ATGP0661-50, ATGP0661-100, ATGP0661-250, ATGP0661-500, ATGP0661-1
References & Citations
Burling F.T., et al. (2003) Acta Crystallogr. 59:73-76; ; Walton L., et al (1989) Nucleic Acids Res. 17 (16) :6741.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_418275
Uniprot Accession Number
P12758
Species
E.coli
AA Sequence
MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL

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