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Recombinant E.coli dsbG protein

CAT: 0112-ATGP0281-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0281-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25% the level of DsbC. Recombinant DsbG was expressed in E. coli and purified by conventional chromatography techniques.
Product Name Alternative
Thiol:disulfide interchange protein dsbG, ybdP, Thiol:disulfide interchange protein dsbG ECK0598, JW0597, Periplasmic disulfide isomerase/thiol disulphide oxidase
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.8 kDa (232aa) confirmed by MALDI-TOF
Additionnal Information
DsbG, Thiol:disulfide interchange protein dsbG, ybdP, Thiol:disulfide interchange protein dsbG ECK0598, JW0597, Periplasmic disulfide isomerase/thiol disulphide oxidase, ATGP0281-10 µg, ATGP0281-20 µg, ATGP0281-50 µg, ATGP0281-100 µg, ATGP0281-250 µg, ATGP0281-500 µg, ATGP0281-1 mg, ATGP0281-10, ATGP0281-20, ATGP0281-50, ATGP0281-100, ATGP0281-250, ATGP0281-500, ATGP0281-1
References & Citations
Riley M., et al (2006) Nucleic Acids Res. 34 (1) :1-9.; ; Andersen CL., et al (1997) Mol Microbiol. 26 (1) :121-32;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_415137
Uniprot Accession Number
P77202
Species
E.coli
AA Sequence
MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK

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