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Recombinant E.coli trxA protein

CAT: 0112-ATGP0549-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0549-010Size:10 µg
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Description
Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant E. coli TXN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Product Name Alternative
Thioredoxin 1, TrxA1, TrxA2
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular Weight
12.8 kDa (117aa) confirmed by MALDI-TOF
Additionnal Information
Thioredoxin1, dasC, fipA, tsnC, ATGP0549-10 µg, ATGP0549-20 µg, ATGP0549-50 µg, ATGP0549-100 µg, ATGP0549-250 µg, ATGP0549-500 µg, ATGP0549-1 mg, ATGP0549-010, ATGP0549-020, ATGP0549-050, ATGP0549-100, ATGP0549-250, ATGP0549-500, ATGP0549-01M
References & Citations
Holmgren A., et al. (1989) J Biol Chem. 264 (24) :13963-6. ; ; Hacquot JP., et al. (1990) Biochem Biophys Res Commun. 173 (3) :1375-81.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Redox Proteins
NCBI Accession Number
ACB04810
Uniprot Accession Number
W8TGE9
Species
E.coli
AA Sequence
MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLA

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