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Recombinant e.coli ackA protein

CAT: 0112-ATGP2844-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2844-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AckA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E. coli ackA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Acetate kinase A and propionate kinase 2, ECK2290, JW2293
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.7 kDa (423aa) confirmed by MALDI-TOF
Additionnal Information
AckA, Acetate kinase A and propionate kinase 2, ECK2290, JW2293, ATGP2844-10 µg, ATGP2844-20 µg, ATGP2844-50 µg, ATGP2844-100 µg, ATGP2844-250 µg, ATGP2844-500 µg, ATGP2844-1 mg, ATGP2844-10, ATGP2844-20, ATGP2844-50, ATGP2844-100, ATGP2844-250, ATGP2844-500, ATGP2844-1
References & Citations
Blattner, F.R., et al. (1997) Science 277 (5331), 1453-1462.; ; Riley, M., et al. (2006) Nucleic Acids Res. 34 (1), 1-9
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416799
Uniprot Accession Number
P0A6A3
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA

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