Recombinant e.coli ackA protein

• Description :

  AckA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E. coli ackA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative :

  Acetate kinase A and propionate kinase 2, ECK2290, JW2293

• Expression System :

  E.coli

• Antigen Species :

  E.coli

• Tag :

  His-Tag

• Applications :

  SDS-PAGE

• Concentration :

  1 mg/mL (determined by Bradford assay)

• Purity :

  > 95% by SDS-PAGE

• Molecular Weight :

  45.7 kDa (423aa) confirmed by MALDI-TOF

• Additionnal Information :

  AckA, Acetate kinase A and propionate kinase 2, ECK2290, JW2293, ATGP2844-10 µg, ATGP2844-20 µg, ATGP2844-50 µg, ATGP2844-100 µg, ATGP2844-250 µg, ATGP2844-500 µg, ATGP2844-1 mg, ATGP2844-10, ATGP2844-20, ATGP2844-50, ATGP2844-100, ATGP2844-250, ATGP2844-500, ATGP2844-1

• References & Citations :

  Blattner, F.R., et al. (1997) Science 277 (5331), 1453-1462.; ; Riley, M., et al. (2006) Nucleic Acids Res. 34 (1), 1-9

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

• Scientific Category :

  Enzymes & Proteases

• NCBI Accession Number :

  NP_416799

• Uniprot Accession Number :

  P0A6A3

• Species :

  E.coli

• AA Sequence :

  MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA