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Recombinant E.coli ung protein

CAT: 0112-ATGP2365-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2365-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ung, also known as uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. uracil bases occur from cytosine deamination or misincorporation of duMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E. coli ung protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Uracil-DNA-glycosylase, ECK2578, JW2564, UDG, UNG1, UNG2, HIGM4
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
28.1 kDa (252aa) confirmed by MALDI-TOF
Additionnal Information
Ung, uracil-DNA-glycosylase, ECK2578, JW2564, ATGP2365-10 µg, ATGP2365-20 µg, ATGP2365-50 µg, ATGP2365-100 µg, ATGP2365-250 µg, ATGP2365-500 µg, ATGP2365-1 mg, ATGP2365-10, ATGP2365-20, ATGP2365-50, ATGP2365-100, ATGP2365-250, ATGP2365-500, ATGP2365-1
References & Citations
Lindahl T. et al. (1977) J Biol Chem. 252 : 3286-3294.; ; Pearl, L. H. et al. (2000) Mutation research 460 : 165-181.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_417075
Uniprot Accession Number
P12295
Species
E.coli
AA Sequence
MANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE

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