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Recombinant e.coli gor protein

CAT: 0112-ATGP1423-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1423-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond. Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 60 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.
Product Name Alternative
Glutathione oxidoreductase, ECK3485, gorA, JW3467
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 60unit/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.
Molecular Weight
51.2 kDa (473aa) confirmed by MALDI-TOF
Additionnal Information
Gor, Glutathione oxidoreductase, ECK3485, gorA, JW3467, ATGP1423-10 µg, ATGP1423-20 µg, ATGP1423-50 µg, ATGP1423-100 µg, ATGP1423-250 µg, ATGP1423-500 µg, ATGP1423-1 mg, ATGP1423-10, ATGP1423-20, ATGP1423-50, ATGP1423-100, ATGP1423-250, ATGP1423-500, ATGP1423-1
References & Citations
Staal G.E. et al. (1969) Biochim. Biophys. Acta 185: 63-69.; ; Stoll V.S.. et al. (1997) Biochemistry 36: 6437-6447.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_417957
Uniprot Accession Number
P06715
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR

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