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Recombinant e.coli glsA1 protein

CAT: 0112-ATGP1053-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1053-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E. coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glutaminase1, ybaS
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.0 kDa (330aa) confirmed by MALDI-TOF
Additionnal Information
GlsA1, Glutaminase1, ybaS, ATGP1053-10 µg, ATGP1053-20 µg, ATGP1053-50 µg, ATGP1053-100 µg, ATGP1053-250 µg, ATGP1053-500 µg, ATGP1053-1 mg, ATGP1053-10, ATGP1053-20, ATGP1053-50, ATGP1053-100, ATGP1053-250, ATGP1053-500, ATGP1053-1
References & Citations
Brown G., et al. (2008) Biochemistry. 47 (21) :5724-35. ; ; Nandakumar R., et al. (2003) J. Mol. Catal. B: Enzymes. 23:87-100.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_415018.1
Uniprot Accession Number
P77454
Species
E.coli
AA Sequence
MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG

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