Products for Research Use Only
RegisterLogin

Recombinant e.coli nth protein

CAT: 0112-ATGP1052-010Size: 10 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGP1052-010Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Endonuclease III, also known as nth, is a DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Recombinant E. coli nth protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Endonuclease III, b1633, JW1625, DNA- (apurinic or apyrimidinic site) lyase, Endonuclease-III
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.7 kDa (231aa) confirmed by MALDI-TOF
Additionnal Information
Nth, Endonuclease III, b1633, JW1625, DNA- (apurinic or apyrimidinic site) lyase, Endonuclease-III, ATGP1052-10 µg, ATGP1052-20 µg, ATGP1052-50 µg, ATGP1052-100 µg, ATGP1052-250 µg, ATGP1052-500 µg, ATGP1052-1 mg, ATGP1052-10, ATGP1052-20, ATGP1052-50, ATGP1052-100, ATGP1052-250, ATGP1052-500, ATGP1052-1
References & Citations
Cunningham R.P., et al. (1994) Acad. Sci. 726:215-222; ; Asahara H., et al. (1989) Biochemistry. 28:4444-4449
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416150
Uniprot Accession Number
P0AB83
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I

Related Products

CatalogName
NUS0801-020Recombinant E.coli NusA protein
HSP0602-500Recombinant E.coli dsbC protein
HSP0602-100Recombinant E.coli dsbC protein
GRL3001-100Recombinant E.coli GroEL protein
DNJ3001-100Recombinant E.coli DnaJ protein
ATGP3752-020Recombinant E.coli dsbC protein
ATGP3239-004Recombinant e.coli idnk protein
ATGP3233-050Recombinant E.coli glk protein
ATGP3233-010Recombinant E.coli glk protein
ATGP3130-004Recombinant e.coli gldA protein

Alternative Products

CatalogName
QP10644-10ugRecombinant E.coli GLDA Protein, His, E.coli-10ug
QP12522-HIS-5ugRecombinant E.coli LACTB Protein, His, E.coli-5ug
QP10894-5ugRecombinant E.coli TRXR Protein, Untagged, E.coli-5ug
QP8857-ec-10ugRecombinant E.coli ADK Protein, His, E.coli-10ug
QP12522-1mgRecombinant E.coli LACTB Protein, Untagged, E.coli-1mg
QP13764-1mgRecombinant E.coli TNAA Protein, His, E.coli-1mg
QP10939-20ugRecombinant E.coli ACKA Protein, His, E.coli-20ug
QP11027-20ugRecombinant E.coli ANSA Protein, His, E.coli-20ug
QP11477-1mgRecombinant E.coli COAA Protein, His, E.coli-1mg
QP11027-1mgRecombinant E.coli ANSA Protein, His, E.coli-1mg

Popular Products