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Recombinant e.coli cysH protein

CAT: 0112-ATGP1041-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1041-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3', 5'-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3'-phosphoadenylyl sulfate and thioredoxin. Recombinant E. coli cysH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Phosphoadenosine phosphosulfate reductase
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30.1 kDa (264aa) confirmed by MALDI-TOF
Additionnal Information
CysH, Phosphoadenosine phosphosulfate reductase, ATGP1041-10 µg, ATGP1041-20 µg, ATGP1041-50 µg, ATGP1041-100 µg, ATGP1041-250 µg, ATGP1041-500 µg, ATGP1041-1 mg, ATGP1041-10, ATGP1041-20, ATGP1041-50, ATGP1041-100, ATGP1041-250, ATGP1041-500, ATGP1041-1
References & Citations
Berendt u., et al. (1995) Eur. J. Biochem. 233 (1) : 347-56.; ; Krone F.A., et al. (1990) FEBS Lett. 260:6-9
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_417242
Uniprot Accession Number
P17854
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG

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