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Recombinant e.coli fimC protein

CAT: 0112-ATGP0931-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0931-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Periplasmic chaperone required for type 1 fimbriae, Periplasmic chaperone, required for type 1 fimbriae, ECK4307, JW4279
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25 kDa (226aa) confirmed by MALDI-TOF
Additionnal Information
FimC, Periplasmic chaperone required for type 1 fimbriae, Periplasmic chaperone, required for type 1 fimbriae, ECK4307, JW4279, ATGP0931-10 µg, ATGP0931-20 µg, ATGP0931-50 µg, ATGP0931-100 µg, ATGP0931-250 µg, ATGP0931-500 µg, ATGP0931-1 mg, ATGP0931-10, ATGP0931-20, ATGP0931-50, ATGP0931-100, ATGP0931-250, ATGP0931-500, ATGP0931-1
References & Citations
Hermanns u., et al. (2000) Biochemistry. 39 (38) :11564-70.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_418736
Uniprot Accession Number
P31697
Species
E.coli
AA Sequence
GVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME

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