A427 Lysate

• Description :

  A427 cells are derived from human lung epithelial carcinoma taken from a 52 year-old male in 1973.

• Antigen Species :

  Human

• Applications :

  WB

• Concentration :

  2 mg/mL (determined by BCA assay)

• Additionnal Information :

  A427, ATGL0028-10 µg, ATGL0028-20 µg, ATGL0028-50 µg, ATGL0028-100 µg, ATGL0028-250 µg, ATGL0028-500 µg, ATGL0028-1 mg, ATGL0028-10, ATGL0028-20, ATGL0028-50, ATGL0028-100, ATGL0028-250, ATGL0028-500, ATGL0028-1

• Storage Conditions :

  Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

• Scientific Category :

  Lysates

• Species :

  Human

• WB Description :

  All lanes : A427 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

• Preparation :

  Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

• Lysate QC :

  Western Blot analyzed by both GAPDH and Beta tubulin antibody