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WiDr Lysate

CAT: 0112-ATGL0020-100Size: 100 µgDry Ice: NoHazardous: No
CAT#:0112-ATGL0020-100Size:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
WiDr cells are established from a 78 year old female with colon adenocarcinoma. The cells are negative for colon antigen 3 expression and positive for keratin by immunoperoxidase staining. WiDr cells expressed p53 antigen. Growth of WiDR cells is inhibited by tumor necrosis factor alpha (TNF-alpha) . Inhibitors of dihydrofolate reductase are highly cytotoxic to WiDR cells.
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
WiDr, ATGL0020-10 µg, ATGL0020-20 µg, ATGL0020-50 µg, ATGL0020-100 µg, ATGL0020-250 µg, ATGL0020-500 µg, ATGL0020-1 mg, ATGL0020-10, ATGL0020-20, ATGL0020-50, ATGL0020-100, ATGL0020-250, ATGL0020-500, ATGL0020-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : WiDr Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody

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