HepG2 Lysate

CAT: 0112-ATGL0005-100Size: 100 µgDry Ice: NoHazardous: No
CAT#:0112-ATGL0005-100Size:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HepG2 cells are immortalized cell line that derived from liver tissue of a 15-year-old boy with hepatocellular carcinoma. HepG2 cells secrete a variety of major plasma proteins such as albumin, transferrin, and secrete acute-phase proteins such as fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin, and plasminogen. HepG2 have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies.
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
HepG2, ATGL0005-10 µg, ATGL0005-20 µg, ATGL0005-50 µg, ATGL0005-100 µg, ATGL0005-250 µg, ATGL0005-500 µg, ATGL0005-1 mg, ATGL0005-10, ATGL0005-20, ATGL0005-50, ATGL0005-100, ATGL0005-250, ATGL0005-500, ATGL0005-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : HepG2 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody

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