293T Lysate

• Description:

  293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein.

• Antigen Species:

  Human

• Applications:

  WB

• Concentration:

  2 mg/mL (determined by BCA assay)

• Additionnal Information:

  293T, ATGL0002-10 µg, ATGL0002-20 µg, ATGL0002-50 µg, ATGL0002-100 µg, ATGL0002-250 µg, ATGL0002-500 µg, ATGL0002-1 mg, ATGL0002-10, ATGL0002-20, ATGL0002-50, ATGL0002-100, ATGL0002-250, ATGL0002-500, ATGL0002-1

• Other References:

  Mizuno R, et al. Expression and localization of anti-Müllerian hormone and its receptors in bovine corpus luteum. (Theriogenology. 2024) {https://pubmed.ncbi.nlm.nih.gov/38924892/}

• Storage Conditions:

  Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

• Scientific Category:

  Lysates

• Species:

  Human

• WB Description:

  All lanes : 293T Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

• Preparation:

  Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

• Lysate QC:

  Western Blot analyzed by both GAPDH and Beta tubulin antibody