Ramos Lysate

CAT: 112-ATGL0010-200Size: 200 µgDry Ice: NoHazardous: No
200 µgSelected
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ramos cells are derived from a Burkitt's lymphoma which does not possess the Epstein Barr Virus (EBV) genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains. Cells are used as model of B lymphocytes and for apoptosis studies.
Product Name Alternative
Human Burkitt's lymphoma cell line
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
Ramos, ATGL0010-10 µg, ATGL0010-20 µg, ATGL0010-50 µg, ATGL0010-100 µg, ATGL0010-250 µg, ATGL0010-500 µg, ATGL0010-1 mg, ATGL0010-10, ATGL0010-20, ATGL0010-50, ATGL0010-100, ATGL0010-250, ATGL0010-500, ATGL0010-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : Ramos Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody