A431 Lysate

CAT: 0112-ATGL0007-100Size: 100 µgDry Ice: NoHazardous: No
CAT#:0112-ATGL0007-100Size:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85 year old female patient. A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the EGFR. As such they are often used as a positive control for EGFR expression.
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
A431, ATGL0007-10 µg, ATGL0007-20 µg, ATGL0007-50 µg, ATGL0007-100 µg, ATGL0007-250 µg, ATGL0007-500 µg, ATGL0007-1 mg, ATGL0007-10, ATGL0007-20, ATGL0007-50, ATGL0007-100, ATGL0007-250, ATGL0007-500, ATGL0007-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : A431 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody

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