A431 Membrane Lysate

CAT: 0223-XBL-10438Size: 0.1 mgDry Ice: YesHazardous: No
CAT#:0223-XBL-10438Size:0.1 mg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
A431 (Human Epidermoid Carcinoma) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The A431 (Human Epidermoid Carcinoma) cell was frozen in liquid nitrogen immediately after excision and then stored at -70˚ C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated A431 (Human Epidermoid Carcinoma) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated A431 (Human Epidermoid Carcinoma) cell membrane protein is then Western analyzed by either GAPDH or β -actin antibody to confirm there is no signal or very weak signal.
Type
Lysates
Concentration
2 mg/mL or better
Buffer
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Shipping Conditions
Dry Ice
Storage Conditions
Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications Notes
A431 (Epidermoid Carcinoma) cell membrane protein lysate is for use in Western blotting, 10 μ g to 20 μ g per lane is recommended for mini gel.
Tested Applications
WB
Species
Human
Physical Properties
Liquid

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