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Human AGXT antibody

CAT: 0112-ATGA0580-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0580-100Size:100 µL
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Description
The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase.Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used toproduce energy and the basic materials important for cellular activities. AGXT converts a compound calledglyoxylate to glycine, an amino acid that is a building block for making enzymes and other proteins.
Product Name Alternative
Serpin B5, Protease inhibitor 5, SERPINB5, alanine-glyoxylate aminotransferase, AGXT1, PH1, AGT, SPT, AGT1, oxalosis I, primary hyperoxaluria type 1, L-alanine: glyoxylate aminotransferase 1, serine:pyruvate aminotransferase, glycolicaciduria
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant AGXT (330-392aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT2T4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
AGXT, ATGA0580-10 µg, ATGA0580-20 µg, ATGA0580-50 µg, ATGA0580-100 µg, ATGA0580-250 µg, ATGA0580-500 µg, ATGA0580-1 mg, ATGA0580-10, ATGA0580-20, ATGA0580-50, ATGA0580-100, ATGA0580-250, ATGA0580-500, ATGA0580-1
References & Citations
Donini S, et al., (2009) Biochem J 422 (2) :265-272.; ; Grujic D, et al., (2009) Am J Nephrol 29 (2) :86-93.; ; Williams EL, et al., (2009) Hum Mutat 30 (6) :910-917.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_000021
Uniprot Accession Number
P21549
WB Description
The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AGXT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human AGXT protein;
FACS Description
Flow cytometry analysis of AGXT in HeLa cells. The cell was stained with ATGA0580 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of AGXT in Hep3B cells. The cell was stained with ATGA0580 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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