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Human ACAT1 antibody

CAT: 0112-ATGA0583-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0583-050Size:50 µL
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Description
ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction. Defects in ACAT1 are a cause of 3-ketothiolase deficiency. 3-ketothiolase deficiency is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded.
Product Name Alternative
Acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ACAT1 (34-427aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
2C5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ACAT1, ATGA0583-10 µg, ATGA0583-20 µg, ATGA0583-50 µg, ATGA0583-100 µg, ATGA0583-250 µg, ATGA0583-500 µg, ATGA0583-1 mg, ATGA0583-10, ATGA0583-20, ATGA0583-50, ATGA0583-100, ATGA0583-250, ATGA0583-500, ATGA0583-1
References & Citations
Antonenkov, V.D., et al. (2000) Eur J Biochem 267: 2981-2990.; ; Korman, S.H. (2006) Mol Genet Metab 89: 289-299.; ; Yamaguchi, S., et al. (1988) J Clin Invest 81 (3) : 813-817.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_000010
Uniprot Accession Number
P24752
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Hep3B cell lysate; ; Lane 4.: 293T cell lysate; ; Lane 5.: A431 cell lysate;
IF Description
ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained with ATGA0583 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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