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Human PARP2 antibody

CAT: 0112-ATGA0578-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0578-050Size:50 µL
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Description
PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable ofcatalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to thatof poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminalcatalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of thisprotein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targetingof the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
Product Name Alternative
Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PARP2 (233-583aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT29G4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PARP2, ATGA0578-10 µg, ATGA0578-20 µg, ATGA0578-50 µg, ATGA0578-100 µg, ATGA0578-250 µg, ATGA0578-500 µg, ATGA0578-1 mg, ATGA0578-10, ATGA0578-20, ATGA0578-50, ATGA0578-100, ATGA0578-250, ATGA0578-500, ATGA0578-1
References & Citations
Ame JC, Rolli V, Schreiber V et al. (1999) . J. Biol. Chem. 274 (25) : 17860-8.; ; Schreiber V, Ame JC, Dolle P et al. (2002) . J. Biol. Chem. 277 (25) : 23028-36.; ; Maeda Y, Hunter TC, Loudy DE et al. (2006) . J. Biol. Chem. 281 (14) : 9600-6.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
NP_005475
Uniprot Accession Number
Q9UGN5
WB Description
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: SW480 cell lysate; ; The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; {ATGA0578-WB2.jpg}The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: NIH3T3 cell lysate; ; Lane 1.: Raji cell lysate; ; {ATGA0578-WB3.jpg}
FACS Description
Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0578 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0578 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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