Human BAK antibody

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP) . BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

BCL2-antagonist/killer 1, Apoptosis regulator BAK, BAK, BAK-LIKE, Bcl-2 homologous antagonist/killer, Bcl2-L-7, BAK-1, BAK 1, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK

Human

Human

Recombinant human BAK (29-187aa) purified from E.coli

AT38E2

Unconjugated

ELISA, WB, ICC/IF, FACS

1 mg/mL (determined by BCA assay)

BAK, ATGA0523-10 µg, ATGA0523-20 µg, ATGA0523-50 µg, ATGA0523-100 µg, ATGA0523-250 µg, ATGA0523-500 µg, ATGA0523-1 mg, ATGA0523-10, ATGA0523-20, ATGA0523-50, ATGA0523-100, ATGA0523-250, ATGA0523-500, ATGA0523-1

Birgit Kemmerling, et al. (2008) Plant Signal Behav, 3 (2) : 116-118.; ; Libo Shan, et al. (2008) Cell Host Microbe, 4 (1) : 17-27.; ; Xu-Bao Shi, et al. (2007) Proc Natl Acad Sci U S A, 104 (50) : 19983-19988.

Singh A, et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. (Front Immunol. 2017) {https://pubmed.ncbi.nlm.nih.gov/28289414/}; ; Mebratu YA, et al. Bik reduces hyperplastic cells by increasing Bak and activating DAPk1 to juxtapose ER and mitochondria. (Nat Commun. 2017) {https://pubmed.ncbi.nlm.nih.gov/28986568/}; ; Liu P, et al. Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1. (Iran J Basic Med Sci. 2017) {https://pubmed.ncbi.nlm.nih.gov/29299204/}; ; Loo LSW, et al. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. (Cell Death Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/32424151/}; ; Shen J, et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. (Diabetol Metab Syndr. 2022) {https://pubmed.ncbi.nlm.nih.gov/35120573/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_001179

Q16611

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : HeLa cell lysate; ; Lane 3 : A431 cell lysate; ; Lane 4 : A549 cell lysate; ; Lane 5 : Jurkat cell lysate ; ; Lane 6 : MCF7 cell lysate ; ; Lane 7 : PC3 cell lysate

Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ATGA0523 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .