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Human PCNA antibody

CAT: 0112-APC0828-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-APC0828-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.
Product Name Alternative
Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PCNA (1-261aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG3 κ
Clone
K1A3
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PCNA, APC0828-10 µg, APC0828-20 µg, APC0828-50 µg, APC0828-100 µg, APC0828-250 µg, APC0828-500 µg, APC0828-1 mg, APC0828-10, APC0828-20, APC0828-50, APC0828-100, APC0828-250, APC0828-500, APC0828-1
References & Citations
Moldovan GL, et al., (2007) Cell 18 129 (4) :665-679.; ; Matsumoto K , et al., (1987) EMBO 6 (3) :637-642.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002583
Uniprot Accession Number
P12004
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCNA (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: NIH3T3 cell lysate

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