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Recombinant human PCNA protein

CAT: 0112-PDN0801-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-PDN0801-100Size:100 µg
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Description
PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by uV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.
Product Name Alternative
Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
28.7 kDa (261aa) confirmed by MALDI-TOF
Additionnal Information
PCNA, Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA, PDN0801-10 µg, PDN0801-20 µg, PDN0801-50 µg, PDN0801-100 µg, PDN0801-250 µg, PDN0801-500 µg, PDN0801-1 mg, PDN0801-10, PDN0801-20, PDN0801-50, PDN0801-100, PDN0801-250, PDN0801-500, PDN0801-1
References & Citations
Matsumoto K., et al. (1987) EMBO J. 6 (3) :637-42. ; ; Moldovan GL., et al. (2007) . Cell. 129 (4) :665-79.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 20% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002583.1
Uniprot Accession Number
P12004
Species
Human
AA Sequence
MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S

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