Products for Research Use Only
RegisterLogin

Human FLIP antibody

CAT: 0112-AFL0926-050Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-AFL0926-050Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1) .
Product Name Alternative
CASP8 and FADD-like apoptosis regulator isoform 1, CFLAR, CASH, CASP8AP1, CLARP, MRIT, Caspase homolog, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human FLIP (1-480aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT8B12
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
FLIP, AFL0926-10 µg, AFL0926-20 µg, AFL0926-50 µg, AFL0926-100 µg, AFL0926-250 µg, AFL0926-500 µg, AFL0926-1 mg, AFL0926-10, AFL0926-20, AFL0926-50, AFL0926-100, AFL0926-250, AFL0926-500, AFL0926-1
References & Citations
Yu JW, Shi Y., (2008) Oncogene. 27 (48) :6216-27.; ; Du C, et al., (2005) Kidney Int. 67 (4) :1397-409.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Apoptosis
NCBI Accession Number
NP_003870
Uniprot Accession Number
O15519
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FLIP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: K562 cell lysate
IF Description
ICC/IF analysis of FLIP in HeLa cells. The cell was stained with AFL0926 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

Related Products

CatalogName
ATGP1195-100Recombinant human FLIP protein
ATGP1195-500Recombinant human FLIP protein
ATGP1195-020Recombinant human FLIP protein
ATGA0604-100Human AGXT antibody
ATGA0583-050Human ACAT1 antibody
ATGA0581-050Human LSM2 antibody
ATGA0580-050Human AGXT antibody
ATGA0578-100Human PARP2 antibody
ATGA0578-050Human PARP2 antibody
ATGA0577-050Human PSMD11 antibody

Alternative Products

CatalogName
ALX-804-961-0100FLIP (human) monoclonal antibody (7F10)
HT233014-01Anti-Human FLIP/CFLAR Polyclonal Antibody
PA87060HU-01Rabbit Anti-Human c-FLIP Polyclonal Antibody
RQ4452FLIP Antibody / c-FLIP
MBS8504402-01FLIP Antibody

Popular Products