Products for Research Use Only

HCM024 Blood Agar Base

CAT: 0938-HCM024Size: 500 g/bottleDry Ice: NoHazardous: No
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CAT#:0938-HCM024Size:500 g/bottle
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Assay Principle
Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant. Formulation (per liter) : Peptone: 10g Beef extract powder :10g Sodium chloride: 5g Agar :15g Final pH 7.4 ± 0.2
Assay Protocol
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, , heated to boiling stirring until completely dissolved, dispensing into flask, 121 °C autoclaved 15min. 2.After the medium is cooled to about 50 °C, sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare. 3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time. 4. Observe the results.
Storage Conditions
Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Quality Control
The following were inoculated after 36 ± 1 °C for 24h , the results are as follows: bacteria name bacteria No. growth status colony characteristics Escherichia coli ATCC25922 not good hemolysis Staphylococcus aureus, ATCC6538 good β-hemolytic Beta-hemolytic streptococcus CMCC (B) 32210 good β-hemolytic Streptococcus pneumoniae CMCC (B) 31001 good α-hemolytic
Recommended Usage
For the growth of pathogenic and non-pathogenic microorganisms.