BRAF Mouse Monoclonal Antibody

• Background:

  BRAF (V-raf murine sarcoma viral oncogene homolog B1) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439) .B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

• Product Name Alternative:

  BRAF

• Gene Name:

  BRAF

• Gene ID:

  673

• Swiss Prot:

  P15056

• Host:

  Mouse

• Reactivity:

  Human

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Applications:

  IHC, ELISA

• Field of Research:

  MAPK signaling pathway

• Purification:

  Affinity Purification

• Dilution:

  IHC 1:200-1:1000, ELISA 1:5000-1:20000

• Form:

  Liquid

• Buffer:

  Ascitic fluid containing 0.03% sodium azide.

• Modification:

  Unmodified

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Isotype:

  Mouse IgG1