Phospho-BRAF (Thr401) Rabbit Polyclonal Antibody

• Background:

  Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

• Product Name Alternative:

  BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

• Gene Name:

  BRAF

• Gene ID:

  673

• Swiss Prot:

  P15056

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, ICC/IF, IP

• Field of Research:

  Cell Biology

• Purification:

  Affinity Chromatography

• Dilution:

  WB 1:500-1:1000, ICC/IF 1:50-1:200, IP 1:20-1:50

• Form:

  Liquid

• Buffer:

  Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phosphorylated

• Molecular Weight:

  Calculated MW: 84 kDa; Observed MW: 84 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG