TRAIL R-1/DR4, soluble

• Description:

  TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain, " which can activate the cell's apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L. Recombinant human soluble TRAIL Receptor-1/DR4 is a 22.7 kDa protein (215 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain.

• Synonyms:

  TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1

• NCBI Gene ID:

  8797

• UniProt:

  O00220

• Accession Number:

  NP_003835.3

• Accession Number mRNA:

  NM_003844.3

• Chromosomal Location:

  8p21

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MSGTGAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASQQLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHN

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Measured by its ability to inhibit apoptosis in LN-18 cells. The expected ED50 for this effect is 0.4 -0.5 µg/ml.

• Length:

  215

• Form:

  Lyophilized

• Molecular Weight:

  22.7 kDa

• Host or Source:

  E. coli