TNF-beta

• Description :

  TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, TNF-β is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF-β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells. Recombinant human TNF-β is a secreted 171 amino acid protein (18.6 kDa) which forms an active non-disulfide linked homotrimer structure in solution.

• Synonyms :

  LTA; LT; TNFB; TNFSF1

• NCBI Gene ID :

  4049

• UniProt :

  P01374

• Accession Number :

  NP_001153212.1

• Accession Number mRNA :

  NM_001159740.1

• Chromosomal Location :

  6p21.3

• Reactivity :

  Human

• Cross Reactivity :

  Mouse, Human

• Sequence :

  MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity :

  The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

• Length :

  171

• Form :

  Lyophilized

• Molecular Weight :

  18.6 kDa

• Host or Source :

  E. coli