TNF-alpha

• Description:

  TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant rat TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

• Synonyms:

  Tnf; Tnfa; RATTNF; TNF-alpha

• NCBI Gene ID:

  24835

• UniProt:

  P16599

• Accession Number:

  NP_036807

• Accession Number mRNA:

  NM_012675

• Chromosomal Location:

  20p12

• Reactivity:

  Anti-Rat

• Cross Reactivity:

  Rat

• Target Antigen:

  Recombinant rat TNF-alpha

• Clone:

  Rabbit IgG

• Applications:

  ELISA, WB, N, IHC

• Purification Method:

  Antigen-affinity purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Form:

  Lyophilized from PBS

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

• Host or Source:

  Rabbit